MALDI-TOF mass spectrometry (MS) is a potentially useful tool for the detection of antimicrobial resistance, especially of that conferred by β-lactamases. Here we describe a modification of a previously reported MALDI-TOF MS meropenem hydrolysis assay. The modified method was validated on 108 carbapenemase-producing Enterobacteriaceae, two NDM-1-producing Acinetobacter baumannii and 35 carbapenem-resistant enterobacteria producing no carbapenemase. The detection of carbapenemases by MALDI-TOF MS seems to be a powerful, quick and cost-effective method for microbiological laboratories.
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